Development of an ovarian biopsy technique in the sea bass, Lates calcarifer (Bloch).
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A convenient and rapid biopsy method for taking ovarian samples from mature sea bass (Lates calcarifer Bloch) is described. Intra-ovarian oocytes siphoned with polyethylene tubing from any region of the ovary provide a sample representative of the maturational stage of sea bass. The osmolality of a 5% phosphate buffered formalin solution is similar to that of sea bass plasma. The follicular diameter of cannulated sea bass oocytes can be measured within an hour after fixation in 5% phosphate buffered formalin without significantly deviating from the diameter of fresh oocytes.
Suggested CitationGarcia, L. (1989). Development of an ovarian biopsy technique in the sea bass, Lates calcarifer (Bloch).
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Kungvankij, Pinij; Tiro Jr., Leonardo B.; Pudadera Jr., Beato J.; Potestas, Ihra O. (Network of Aquaculture Centres in Asia, 1986)Information is presented regarding the biology and culture of Lates calcarifer in order to serve as a practical guide for extension workers and farmers. Hatchery design, seed production and hatchery techniques, culture of ...
Parazo, Monina M.; Garcia, LM; Ayson, Felix G.; Fermin, Armando C.; Almendras, Jesus M. E.; Reyes Jr., Deogracias M.; Avila, Enrique M.; Toledo, Joebert D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 1998)The manual addresses sea bass (Lates calcarifer) fry production in the hatchery. It describes the principles and practical procedures for rearing sea bass - from eggs until metamorphosis - as practiced by the SEAFDEC ...